The EPO ELISA Kit is designed for the quantitative detection of Erythropoietin (EPO) levels in various human biological samples. EPO is a crucial hormone involved in the regulation of red blood cell production. It plays a significant role in the body's response to hypoxia and has been implicated in managing anemia in chronic kidney disease, cancer, and other health conditions.
Accurate measurement of EPO is crucial for understanding its role in erythropoiesis and for developing targeted therapeutic strategies. Assay Genie's EPO ELISA Kit offers outstanding sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's EPO ELISA Kit for accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Human EPO (Erythropoietin) ELISA Kit
SKU:
AEES00038
Target:
Human EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EPO. You can calculate the concentration of Human EPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
91-103
95-106
Average (%)
103
95
103
1:4
Range (%)
92-100
91-105
100-108
Average (%)
97
99
104
1:8
Range (%)
94-110
97-106
96-104
Average (%)
102
103
99
1:16
Range (%)
96-103
85-99
95-109
Average (%)
99
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-109
105
EDTA plasma (n=5)
100-113
105
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.28
12.36
43.07
4.12
10.77
43.71
Standard deviation
0.22
0.62
2.33
0.21
0.49
1.95
C V (%)
5.14
5.02
5.41
5.1
4.55
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPO in samples. No significant cross-reactivity or interference between Human EPO and analogues was observed.
