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Human DBH Recombinant Protein (RPPB1555)

SKU:
RPPB1555
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P09172
Research Area:
Enzymes
€309
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Description

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Product Name:Human DBH Recombinant Protein
Product Code:RPPB1555
Size:10µg
Species:Human
Target:DBH
Synonyms:EC 1.14.17.1, DBM, DBH.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:DBH protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSAPRESPLP YHIPLDPEGS LELSWNVSYT QEAIHFQLLV RRLKAGVLFG MSDRGELENA DLVVLWTDGD TAYFADAWSD QKGQIHLDPQ QDYQLLQVQR TPEGLTLLFK RPFGTCDPKD YLIEDGTVHL VYGILEEPFR SLEAINGSGL QMGLQRVQLL KPNIPEPELP SDACTMEVQA PNIQIPSQET TYWCYIKELP KGFSRHHIIK YEPIVTKGNE ALVHHMEVFQ CAPEMDSVPH FSGPCDSKMK PDRLNYCRHV LAAWALGAKA FYYPEEAGLA FGGPGSSRYL RLEVHYHNPL VIEGRNDSSG IRLYYTAKLR RFNAGIMELG LVYTPVMAIP PRETAFILTG YCTDKCTQLA LPPSGIHIFA SQLHTHLTGR KVVTVLVRDG REWEIVNQDN HYSPHFQEIR MLKKVVSVHP GDVLITSCTY NTEDRELATV GGFGILEEMC VNYVHYYPQT QLELCKSAVD AGFLQKYFHL INRFNNEDVC TCPQASVSQQ FTSVPWNSFN RDVLKALYSF APISMHCNKS SAVRFQGEWN LQPLPKVIST LEEPTPQCPT SQGRSPAGPT VVSIGGGKG

DBH catalyzes the chemical reaction. DBH is an oxidoreductase which belongs to the copper type II, ascorbate-dependent monooxygenase family, in particular those performing on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated can not be derived from O2 with reduced ascorbate as one donor, as well as incorporation of one ato of oxygen into the other donor.�

DBH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 599 amino acids (40-617 a.a) and having a molecular mass of 67.2kDa.DBH is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; EC 1.14.17.1; Oxidoreductase; Apoptosis; Amino Acid Metabolism - tyrosine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: membrane; cytoplasm; integral to membrane; extracellular region

Molecular Function:copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; catalytic activity

Biological Process: fear response; dopamine catabolic process; maternal behavior; response to amphetamine; cytokine production; locomotory behavior; response to pain; behavioral response to ethanol; leukocyte mediated immunity; glucose homeostasis; memory; regulation of cell proliferation; norepinephrine biosynthetic process; synaptic transmission; catecholamine biosynthetic process; homoiothermy; positive regulation of vasoconstriction; visual learning; blood vessel remodeling; leukocyte migration

Disease: Dopamine Beta-hydroxylase Deficiency, Congenital

NCBI Summary:The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
UniProt Code:P09172
NCBI GenInfo Identifier:158517849
NCBI Gene ID:1621
NCBI Accession:P09172.3
UniProt Related Accession:P09172
Molecular Weight:
NCBI Full Name:Dopamine beta-hydroxylase
NCBI Synonym Full Names:dopamine beta-hydroxylase
NCBI Official Symbol:DBH��
NCBI Official Synonym Symbols:DBM; ORTHYP1��
NCBI Protein Information:dopamine beta-hydroxylase
UniProt Protein Name:Dopamine beta-hydroxylase
UniProt Synonym Protein Names:Dopamine beta-monooxygenaseSoluble dopamine beta-hydroxylase
Protein Family:Dopamine beta-hydroxylase
UniProt Gene Name:DBH��
UniProt Entry Name:DOPO_HUMAN

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