Human CYB5A Recombinant Protein (RPPB3318)
- SKU:
- RPPB3318
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00167
Description
| Product Name: | Human CYB5A Recombinant Protein |
| Product Code: | RPPB3318 |
| Size: | 10µg |
| Species: | Human |
| Target: | CYB5A |
| Synonyms: | Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CYB5A protein solution (1mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPMAEQSDE AVKYYTLEEI QKHNHSKSTW LILHHKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDAREMSK TFIIGELHPDDRPKLNKPPE TLITTIDSSS SHHHHHH |
Cytochrome b5 isoform 1 or CYB5A, is part of the cytochrome b5 family which is bound to the cell membrane. CYB5A has a key role in the stearyl-CoA-desaturase activity as it reduces ferric hemoglobin to ferrous hemoglobin. Cytochrome b5 isoform 1 performs as an electron carrier for numerous� oxygenases that are imbedded in the cell membrane.�
CYB5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 117 amino acids (1-108a.a.) and having a molecular mass of 13.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).CYB5A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 18q23 Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane Molecular Function:cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process Disease: Methemoglobinemia Type Iv |
| NCBI Summary: | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P00167 |
| NCBI GenInfo Identifier: | 117809 |
| NCBI Gene ID: | 1528 |
| NCBI Accession: | P00167.2 |
| UniProt Secondary Accession: | P00167,Q6IB14, A8MV91, F8WEU4, |
| UniProt Related Accession: | P00167 |
| Molecular Weight: | 14,169 Da |
| NCBI Full Name: | Cytochrome b5 |
| NCBI Synonym Full Names: | cytochrome b5 type A (microsomal) |
| NCBI Official Symbol: | CYB5A�� |
| NCBI Official Synonym Symbols: | CYB5; MCB5�� |
| NCBI Protein Information: | cytochrome b5; type 1 cyt-b5 |
| UniProt Protein Name: | Cytochrome b5 |
| UniProt Synonym Protein Names: | Microsomal cytochrome b5 type A; MCB5 |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYB5A�� |
| UniProt Entry Name: | CYB5_HUMAN |
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