Human CUL1 Recombinant Protein (RPPB3312)
- SKU:
- RPPB3312
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13616
Description
| Product Name: | Human CUL1 Recombinant Protein |
| Product Code: | RPPB3312 |
| Size: | 10µg |
| Species: | Human |
| Target: | CUL1 |
| Synonyms: | Cullin 1, CUL-1, Cullin-1 |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The CUL1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK |
Cullin1, is an essential component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, that facilitate the ubiquitination of proteins which take part in cell cycle progression, signal transduction and transcription. In the SCF complex. Cullin1 assists in a rigid scaffold which organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 contributes to catalysis by positioning of the substrate and the ubiquitin-conjugating enzyme.
CUL1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 430 amino acids (1- 410a.a.) and having a molecular mass of 49.4kDa.CUL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 7q36.1 Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: protein monoubiquitination; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; cell cycle arrest; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| UniProt Code: | Q13616 |
| NCBI GenInfo Identifier: | 19863257 |
| NCBI Gene ID: | 8454 |
| NCBI Accession: | Q13616.2 |
| UniProt Secondary Accession: | Q13616,O60719, Q08AL6, Q8IYW1, D3DWG3, |
| UniProt Related Accession: | Q13616 |
| Molecular Weight: | 89,679 Da |
| NCBI Full Name: | Cullin-1 |
| NCBI Synonym Full Names: | cullin 1 |
| NCBI Official Symbol: | CUL1�� |
| NCBI Protein Information: | cullin-1; CUL-1 |
| UniProt Protein Name: | Cullin-1 |
| Protein Family: | Cullin |
| UniProt Gene Name: | CUL1�� |
| UniProt Entry Name: | CUL1_HUMAN |
