null

Human Chemokine C-C-Motif Receptor 4 (CCR4) ELISA Kit

SKU:
HUDL00470
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51679
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD194,CC-CKR-4,CKR4,CMKBR4,ChemR13,K5-5
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Chemokine C-C-Motif Receptor 4 (CCR4) ELISA Kit

The Human CCR4 ELISA Kit is specifically designed for the precise measurement of CCR4 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research purposes.CCR4, also known as C-C chemokine receptor type 4, is a vital receptor involved in immune response and inflammatory processes. It plays a key role in conditions such as inflammatory diseases, allergies, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.

With the Human CCR4 ELISA Kit, researchers can confidently analyze CCR4 levels in human samples, advancing their understanding of immune responses and inflammatory mechanisms. Order your kit today from Assay Genie and unlock new insights into CCR4-related research.

Product Name:Human Chemokine C-C-Motif Receptor 4 (CCR4) ELISA Kit
Product Code:HUDL00470
Size:96 Assays
Target:CCR4
Synonyms:CD194, CC-CKR-4, CKR4, CMKBR4, ChemR13, K5-5
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products