Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit
The Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit is a powerful tool for measuring CFTR levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for research purposes.CFTR is a key protein involved in the regulation of chloride and water transport in cells, with mutations in the CFTR gene leading to the development of cystic fibrosis.
By measuring CFTR levels, researchers can better understand the molecular mechanisms underlying this genetic disorder and develop potential therapies.This ELISA kit is a valuable resource for studying cystic fibrosis and related conditions, offering a comprehensive solution for quantitative analysis of CFTR levels in human samples. Its ease of use and precision make it an essential tool for any laboratory conducting research in this area.
Product Name:
Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit
SKU:
HUES02743
Target:
Human CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFTR. You can calculate the concentration of Human CFTR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
88-101
85-97
Average (%)
97
94
91
1:4
Range (%)
92-108
82-93
82-98
Average (%)
99
87
89
1:8
Range (%)
85-100
80-92
85-96
Average (%)
92
85
90
1:16
Range (%)
88-100
84-98
82-96
Average (%)
95
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.21
4.55
0.47
1.21
4.66
Standard deviation
0.03
0.07
0.19
0.03
0.05
0.18
C V (%)
6.52
5.79
4.18
6.38
4.13
3.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CFTR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed.
