The Human ApoA2 (Apolipoprotein A2) ELISA Kit is meticulously developed for the quantitative detection of ApoA2 levels in various human biological samples.
Apolipoprotein A2 is a crucial protein involved in lipid metabolism and lipid transport processes, particularly in relation to cholesterol and triglycerides. It plays a significant role in lipid and lipoprotein regulation in the body. Accurate measurement of ApoA2 levels is essential for understanding its role in maintaining lipid homeostasis and its potential implications in cardiovascular health. The ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With robust performance and user-friendly operation, this kit is suitable for both research and clinical applications. Trust the Assay Genie's ApoA2 ELISA Kit for accurate and reliable quantification of this vital biomarker in your studies.
Product Name:
Human ApoA2 (Apolipoprotein A2) ELISA Kit
SKU:
AEES00075
Target:
Human ApoA2 (Apolipoprotein A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA2. You can calculate the concentration of Human ApoA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-108
96-106
88-101
Average (%)
105
100
96
1:4
Range (%)
87-97
92-104
88-98
Average (%)
92
96
92
1:8
Range (%)
97-110
86-94
97-108
Average (%)
105
91
104
1:16
Range (%)
97-107
85-100
95-102
Average (%)
104
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
94
EDTA plasma (n=5)
96-109
101
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.14
224.95
844.46
86.28
245.63
844.5
Standard deviation
5.52
10.12
37.07
5.16
11.45
45.52
C V (%)
5.74
4.5
4.39
5.98
4.66
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA2 in samples. No significant cross-reactivity or interference between Human ApoA2 and analogues was observed.
