The Hexokinase-3 Polyclonal Antibody (CAB8428) is a valuable tool for researchers studying hexokinase-3, an enzyme involved in cellular metabolism and energy production. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is specifically validated for use in Western blot applications. By binding to the hexokinase-3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in biochemistry and cancer research.Hexokinase-3 plays a crucial role in the glycolysis pathway, where it catalyzes the first step in glucose metabolism by converting glucose to glucose-6-phosphate.
Dysregulation of hexokinase-3 has been implicated in various diseases, including cancer and metabolic disorders. Therefore, investigating the function of hexokinase-3 with the help of this antibody is essential for understanding its role in these pathological conditions.With its high specificity and reliability, the Hexokinase-3 Polyclonal Antibody (CAB8428) is a valuable asset for researchers seeking to unravel the complexities of cellular metabolism and its implications in health and disease.
Product Name:
HK3 Rabbit Polyclonal Antibody
SKU:
CAB8428
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Purification Method:
Affinity purification
Gene ID:
3101
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HK3 antibody (CAB8428) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
