The GPT2 Polyclonal Antibody is a crucial tool for researchers studying GPT2, a key enzyme involved in amino acid metabolism and protein synthesis. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blotting and immunohistochemistry applications. By binding to the GPT2 protein, this antibody enables accurate detection and analysis in various cell types, making it essential for studies in biochemistry, metabolism, and cancer research.GPT2, also known as alanine aminotransferase 2, plays a vital role in the breakdown of amino acids and the production of energy in cells.
Dysregulation of GPT2 has been linked to various metabolic disorders and cancer progression, making it a promising target for therapeutic interventions. Research into the function and activity of GPT2 is essential for developing novel treatments for metabolic diseases and cancer, ultimately advancing our understanding of cellular metabolism and disease mechanisms.
Product Name:
GPT2 Polyclonal Antibody
SKU:
CAB23670
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human GPT2.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
ALT2; GPT 2; MRT49; NEDSPM; GPT2
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
mitochondrial matrix
Calculated MW:
58kDa
Observed MW:
58 kDa
This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84706
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HepG2, using GPT2 Rabbit pAb (CAB23670) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
