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GPR12 Antibody (PACO55526)

SKU:
PACO55526
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • GPR12 Antibody PACO55526
  • GPR12 Antibody PACO55526
  • GPR12 Antibody PACO55526
€339
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Description

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GPR12 Antibody (PACO55526)

The GPR12 Polyclonal Antibody (PACO55526) is a valuable tool for researchers studying G protein-coupled receptor 12 (GPR12), a protein involved in various cellular processes, including development, metabolism, and neurological function. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.GPR12 is a receptor located on the cell membrane that interacts with extracellular molecules to transmit signals inside the cell, regulating key physiological functions. Its role in signaling pathways makes it a promising target for investigating conditions such as neurodevelopmental disorders, metabolic diseases, and reproductive health.

By targeting the GPR12 protein, researchers can explore its impact on cellular functions and pathways, providing insights into its potential as a therapeutic target for various diseases. The GPR12 Polyclonal Antibody is a reliable tool for detecting and analyzing GPR12 expression in different cell types, making it an essential component for studies in neuroscience, metabolism, and reproductive biology.

Antibody Name:GPR12 Antibody (PACO55526)
Antibody SKU:PACO55526
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human G-protein coupled receptor 12 protein (1-48AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: K562 whole cell lysate. All lanes: GPR12 antibody at 2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 37 kDa.
Product ImageImmunofluorescence staining of Hela cells with PACO55526 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageIHC image of PACO55526 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity). Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production.
Synonyms:G-protein coupled receptor 12, GPR12
UniProt Protein Function:GPR12: Promotes neurite outgrowth and blocks myelin inhibition in neurons. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integralChromosomal Location of Human Ortholog: 13q12Cellular Component: integral to plasma membraneMolecular Function: G-protein coupled receptor activity; phosphatidylcholine bindingBiological Process: cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway
UniProt Protein Details:
NCBI Summary:
UniProt Code:P47775
NCBI GenInfo Identifier:4885295
NCBI Gene ID:2835
NCBI Accession:NP_005279.1
UniProt Secondary Accession:P47775,Q5T8P3
UniProt Related Accession:P47775
Molecular Weight:36,730 Da
NCBI Full Name:G-protein coupled receptor 12
NCBI Synonym Full Names:G protein-coupled receptor 12
NCBI Official Symbol:GPR12  
NCBI Official Synonym Symbols:GPCR12; GPCR21; PPP1R84  
NCBI Protein Information:G-protein coupled receptor 12; protein phosphatase 1, regulatory subunit 84
UniProt Protein Name:G-protein coupled receptor 12
UniProt Synonym Protein Names:
Protein Family:G-protein coupled receptor
UniProt Gene Name:GPR12  
UniProt Entry Name:GPR12_HUMAN
Antibodies
GPR12 Antibody (PACO03922)
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011)
Anti-HRP-conjugated Beta Actin Antibody (CABC028)

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