The General Vitamin B6 (VB6) ELISA Kit from Assay Genie is a cutting-edge tool for precise and efficient detection of Vitamin B6 levels in various biological samples, including serum, plasma, and tissue extracts. With its outstanding sensitivity and specificity, this kit offers researchers and healthcare professionals a reliable solution for studying the role of Vitamin B6 in various physiological processes and disease states. Vitamin B6, also known as pyridoxine, is an essential nutrient that plays a crucial role in numerous biochemical reactions within the body, including metabolism, neurotransmitter synthesis, and immune function.
Deficiencies in Vitamin B6 have been linked to a range of health issues, such as anemia, cognitive impairment, and cardiovascular disease, underscoring the importance of accurate measurement of Vitamin B6 levels. Whether investigating Vitamin B6 status in clinical settings or conducting basic research on its impact on health and disease, the General Vitamin B6 (VB6) ELISA Kit provides users with a reliable and efficient tool for precise quantification of Vitamin B6 levels. Trust Assay Genie to deliver accurate results and enable groundbreaking discoveries in the field of Vitamin B6 research.
Product Name:
General Vitamin B6 (VB6) ELISA Kit
Product Code:
UNDL00080
Size:
96 Assays
Target:
VB6
Synonyms:
Pyridoxine
Detection Method:
Competition
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
