The GCLM Rabbit Monoclonal Antibody is a valuable tool for researchers studying glutamate-cysteine ligase modifier subunit (GCLM), a key enzyme involved in the biosynthesis of glutathione, an important antioxidant in cells. This antibody, produced using cutting-edge monoclonal antibody technology in rabbits, is highly specific for detecting GCLM in human samples and is ideal for use in Western blot applications.GCLM plays a crucial role in maintaining cellular redox balance and protecting cells from oxidative stress. Dysregulation of GCLM has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
By using this antibody to study GCLM expression and function, researchers can gain insights into the role of this enzyme in disease pathogenesis and explore its potential as a therapeutic target.The GCLM Rabbit Monoclonal Antibody offers high sensitivity and specificity, enabling researchers to accurately detect GCLM levels in cell lysates and tissue samples. By understanding the mechanisms underlying GCLM regulation and function, scientists can advance our knowledge of cellular antioxidant defense systems and develop new strategies for treating diseases associated with oxidative stress.
Product Name:
GCLM Rabbit Monoclonal Antibody
SKU:
CAB11444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 175-274 of human GCLM (P48507).
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
2730
Clone Number:
ARC0597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GCLM antibody (CAB11444) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
