The FITC Anti-Mouse CD45 Antibody (30-F11) from Assay Genie is a powerful tool for researchers studying immune cells and immune responses in mice. This antibody specifically targets the CD45 protein, a key marker found on the surface of leukocytes. By binding to CD45, this antibody allows for precise detection and analysis of immune cell populations in experimental settings.CD45 is essential for immune cell function, playing a crucial role in signal transduction and cell activation. By using the FITC Anti-Mouse CD45 Antibody (30-F11), researchers can accurately identify and characterize different subsets of immune cells, helping to elucidate the complex interactions within the immune system.
This antibody is highly specific for mouse samples and is suitable for a variety of applications, including flow cytometry and immunofluorescence. With its high reactivity and reliable performance, the FITC Anti-Mouse CD45 Antibody (30-F11) is an indispensable tool for studies in immunology, inflammation, and infectious diseases in mouse models.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P06800
Gene ID:
19264
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.