ESRP2 Antibody (PACO56326)
- SKU:
- PACO56326
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
ESRP2 Antibody (PACO56326)
The ESRP2 Polyclonal Antibody (PAC056326) is specifically designed for research involving ESRP2, a key protein involved in alternative splicing regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting. By targeting the ESRP2 protein, this antibody enables accurate detection and analysis in various cell types, making it well-suited for studies in molecular biology and cancer research.ESRP2, also known as Epithelial Splicing Regulatory Protein 2, plays a crucial role in regulating alternative splicing events that impact gene expression and cellular function.
Its involvement in splicing regulation has implications for various biological processes, making it a valuable target for research into mechanisms underlying diseases like cancer and neurological disorders. Investigating the function of ESRP2 is key to advancing our understanding of splicing regulation and its role in disease pathogenesis.
| Antibody Name: | ESRP2 Antibody (PACO56326) |
| Antibody SKU: | PACO56326 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Epithelial splicing regulatory protein 2 protein (615-720AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ESRP2 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 79, 78 kDa. Observed band size: 79 kDa. |
 | IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| Synonyms: | Epithelial splicing regulatory protein 2 (RNA-binding motif protein 35B) (RNA-binding protein 35B), ESRP2, RBM35B |
| UniProt Protein Function: | RBM35B: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: 16q22.1Cellular Component: nucleoplasm; nucleusMolecular Function: mRNA binding; nucleotide bindingBiological Process: regulation of RNA splicing; RNA splicing; mRNA processing |
| UniProt Protein Details: | |
| NCBI Summary: | ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009] |
| UniProt Code: | Q9H6T0 |
| NCBI GenInfo Identifier: | 45935393 |
| NCBI Gene ID: | 80004 |
| NCBI Accession: | NP_079215 |
| UniProt Secondary Accession: | Q9H6T0,Q8N6H8, Q8WZ15, Q9H6I4 |
| UniProt Related Accession: | Q9H6T0 |
| Molecular Weight: | 77,364 Da |
| NCBI Full Name: | epithelial splicing regulatory protein 2 |
| NCBI Synonym Full Names: | epithelial splicing regulatory protein 2 |
| NCBI Official Symbol: | ESRP2Â Â |
| NCBI Official Synonym Symbols: | RBM35BÂ Â |
| NCBI Protein Information: | epithelial splicing regulatory protein 2; RNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B |
| UniProt Protein Name: | Epithelial splicing regulatory protein 2 |
| UniProt Synonym Protein Names: | RNA-binding motif protein 35B; RNA-binding protein 35B |
| Protein Family: | Epithelial splicing regulatory protein |
| UniProt Gene Name: | ESRP2Â Â |
| UniProt Entry Name: | ESRP2_HUMAN |
Related Products
HNR Antibody Antibody (CAB17497)
€139 - €419
Anti-CD123 Biotinylated Recombinant Antibody Antibody (HDLA007)
€1,012