ESRP2 Antibody (PACO56326)
- SKU:
- PACO56326
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | ESRP2 Antibody (PACO56326) |
| Antibody SKU: | PACO56326 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Epithelial splicing regulatory protein 2 protein (615-720AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ESRP2 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 79, 78 kDa. Observed band size: 79 kDa. |
 | IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. |
| Synonyms: | Epithelial splicing regulatory protein 2 (RNA-binding motif protein 35B) (RNA-binding protein 35B), ESRP2, RBM35B |
| UniProt Protein Function: | RBM35B: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: 16q22.1Cellular Component: nucleoplasm; nucleusMolecular Function: mRNA binding; nucleotide bindingBiological Process: regulation of RNA splicing; RNA splicing; mRNA processing |
| UniProt Protein Details: | |
| NCBI Summary: | ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009] |
| UniProt Code: | Q9H6T0 |
| NCBI GenInfo Identifier: | 45935393 |
| NCBI Gene ID: | 80004 |
| NCBI Accession: | NP_079215 |
| UniProt Secondary Accession: | Q9H6T0,Q8N6H8, Q8WZ15, Q9H6I4 |
| UniProt Related Accession: | Q9H6T0 |
| Molecular Weight: | 77,364 Da |
| NCBI Full Name: | epithelial splicing regulatory protein 2 |
| NCBI Synonym Full Names: | epithelial splicing regulatory protein 2 |
| NCBI Official Symbol: | ESRP2Â Â |
| NCBI Official Synonym Symbols: | RBM35BÂ Â |
| NCBI Protein Information: | epithelial splicing regulatory protein 2; RNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B |
| UniProt Protein Name: | Epithelial splicing regulatory protein 2 |
| UniProt Synonym Protein Names: | RNA-binding motif protein 35B; RNA-binding protein 35B |
| Protein Family: | Epithelial splicing regulatory protein |
| UniProt Gene Name: | ESRP2Â Â |
| UniProt Entry Name: | ESRP2_HUMAN |