The EphB4 Polyclonal Antibody (CAB3293) is a valuable tool for researchers studying EphB4, a receptor tyrosine kinase involved in various cellular processes, including cell adhesion, migration, and angiogenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to EphB4, this antibody allows for the detection and analysis of EphB4 protein expression in a range of cell types, making it ideal for investigations in cancer biology and developmental biology.
EphB4 has been implicated in tumor growth, metastasis, and angiogenesis, making it a promising target for cancer therapy. Additionally, EphB4 plays a role in vascular development and neuronal guidance, highlighting its importance in various physiological and pathological processes. Further research into the function of EphB4 may provide insights into potential therapeutic strategies for cancer and other diseases.
Product Name:
EPHB4 Rabbit Polyclonal Antibody
SKU:
CAB3293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-540 of human EPHB4 (NP_004435.3).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
108kDa
Observed MW:
108kDa
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
Purification Method:
Affinity purification
Gene ID:
2050
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse liver, using EPHB4 antibody (CAB3293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
