The DNAH12 Polyclonal Antibody (PACO50674) is a high-quality research tool designed for studying the DNAH12 protein, a key component of ciliary dynein arms essential for proper cilia function. This antibody, raised in rabbits, is specifically reactive with human samples and is validated for use in various applications, including Western blot and immunofluorescence.DNAH12 plays a crucial role in the movement and function of cilia, which are hair-like structures on the surface of cells that play important roles in various physiological processes. Dysfunction of these cilia has been implicated in a range of human diseases, including respiratory disorders, infertility, and developmental disorders.
By targeting the DNAH12 protein, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies.This antibody provides a reliable tool for detecting and analyzing DNAH12 expression in different cell types and tissues, making it ideal for studies in cell biology, developmental biology, and disease research. By understanding the function of DNAH12, researchers can uncover new avenues for investigating cilia-related disorders and advancing our knowledge of cellular function.
Immunofluorescent analysis of HepG2 cells using PACO50674 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot. Positive WB detected in Recombinant protein. All lanes: DNAH12 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 kDa. Observed band size: 53 kDa.
Background:
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly (By similarity).
Synonyms:
Dynein heavy chain 12, axonemal (Axonemal β dynein heavy chain 12) (Axonemal dynein heavy chain 12-like protein) (Axonemal dynein heavy chain 7-like protein) (Ciliary dynein heavy chain 12) (Dynein heavy chain 7-like, axonemal) (Dynein heavy chain domain-containing protein 2), DNAH12, DHC3 DLP12 DNAH12L DNAH7L DNAHC3 DNHD2 HDHC3 HL19
UniProt Protein Function:
DNAHC12: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly. Belongs to the dynein heavy chain family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 3p14.3
Axonemal beta dynein heavy chain 12; Axonemal dynein heavy chain 12-like protein; Axonemal dynein heavy chain 7-like protein; Ciliary dynein heavy chain 12; Dynein heavy chain 7-like, axonemal; Dynein heavy chain domain-containing protein 2
