The DEPP1 Polyclonal Antibody (PAC060336) is a valuable tool for researchers studying the DEPP1 protein, which is involved in various cellular processes, including apoptosis, autophagy, and stress responses. This antibody, produced in rabbits, shows high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to DEPP1, this antibody enables accurate detection and analysis in a wide range of cell types, making it ideal for research in cell biology and cancer biology.
DEPP1, also known as Death Effector Filament-forming Peptide 1, plays a critical role in cell survival and death pathways, making it a key player in determining cell fate under various conditions. Its involvement in regulating cellular responses to stress and promoting cell death makes it a promising target for studying diseases such as cancer and neurodegenerative disorders. Understanding the function of DEPP1 is essential for developing targeted therapies that manipulate cell survival pathways in disease contexts.
IHC image of PACO60336 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO60336 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
May play a role in autophagy.
Synonyms:
Protein DEPP1 (Decidual protein induced by progesterone) (Fasting-induced gene protein) (FIG), DEPP1, C10orf10 DEPP FIG
UniProt Protein Function:
Acts as a critical modulator of FOXO3-induced autophagy via increased cellular ROS.
UniProt Protein Details:
NCBI Summary:
The expression of this gene is induced by fasting as well as by progesterone. The protein encoded by this gene contains a t-synaptosome-associated protein receptor (SNARE) coiled-coil homology domain and a peroxisomal targeting signal. Production of the encoded protein leads to phosphorylation and activation of the transcription factor ELK1. [provided by RefSeq, Jul 2008]
