The DDX54 Polyclonal Antibody (PACO22179) is a valuable tool for researchers studying DDX54, a protein involved in RNA metabolism and cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DDX54 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in fields such as molecular biology and cancer research.DDX54, also known as a DEAD-box RNA helicase, plays a crucial role in RNA processing and regulation, making it a key target for studies on gene expression and cellular function.
Understanding the functions of DDX54 is essential for unraveling its roles in processes such as mRNA splicing, translation, and RNA degradation. Research on DDX54 could provide insights into diseases related to RNA dysregulation, including cancer and neurodegenerative disorders, and may lead to the development of novel therapeutic strategies targeting RNA metabolism pathways.
Antibody Name:
DDX54 Antibody (PACO22179)
Antibody SKU:
PACO22179
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human DDX54.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from CoLo cells, 293 cells and JurKat cells, using DDX54 antibody.
Background:
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Synonyms:
ATP-dependent RNA helicase DDX54; EC=3.6.4.13; ATP-dependent RNA helicase DP97; DEAD box RNA helicase 97 kDa; DEAD box protein 54
UniProt Protein Function:
DDX54: Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors. Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.4.13; Helicase; Transcription, coactivator/corepressor; Nucleolus; RNA processing; Nuclear receptor co-regulatorChromosomal Location of Human Ortholog: 12q24.13Cellular Component: membrane; nucleolus; nucleusMolecular Function: estrogen receptor binding; transcription corepressor activity; ATP-dependent RNA helicase activity; ATP binding; receptor bindingBiological Process: RNA processing; estrogen receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA metabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
