The CPNE8 Polyclonal Antibody (PACO59189) is a valuable tool for researchers studying CPNE8, a calcium-dependent protein involved in various cellular processes, including membrane trafficking and signaling. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CPNE8 protein, this antibody enables the detection and analysis of CPNE8 in different cell types, making it an excellent choice for studies in cell biology and molecular biology.
CPNE8, also known as Copine 8, has been implicated in several biological functions, such as membrane fusion, cell adhesion, and intracellular signaling. Its diverse roles in cellular processes make it a promising target for research in various fields, including neuroscience, cancer biology, and developmental biology. Investigating the functions and mechanisms of CPNE8 can provide insights into its potential involvement in disease pathogenesis and may lead to the development of novel therapeutic strategies targeting CPNE8-related pathways.
IHC image of PACO59189 diluted at 1:600 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO59189 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Probable calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes.
Synonyms:
Copine-8 (Copine VIII), CPNE8
UniProt Protein Function:
CPNE8: a member of the copine protein family with calcium-dependent phospholipid binding properties. Copines are ubiquitously expressed, phospholipid-binding proteins that have been conserved through evolution. Highest expression of CPNE8 mRNA is seen in the prostate, heart, and brain. Fusion to AML1 in a t(12;21) translocation causes aggressive acute myelogenous leukemia. Two alternatively spliced isoforms have been described.Protein type: Unknown functionChromosomal Location of Human Ortholog: 12q12
UniProt Protein Details:
NCBI Summary:
Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encode a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. [provided by RefSeq, Jul 2008]
