The CLPP Polyclonal Antibody (CAB9127) is a high-quality research tool designed for the detection and analysis of CLPP, a mitochondrial protease involved in protein quality control and maintenance of mitochondrial homeostasis. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.CLPP, also known as caseinolytic mitochondrial matrix peptidase proteolytic subunit, plays a crucial role in the regulation of mitochondrial functions, including protein turnover and quality control. Its involvement in maintaining mitochondrial health makes it a key target for research in mitochondrial biology, metabolism, and aging-related diseases.
By binding specifically to the CLPP protein, this polyclonal antibody enables the accurate detection and analysis of CLPP levels in various cell types and tissues. Its utility in studying mitochondrial function and dysfunction makes it a valuable tool for research in areas such as bioenergetics, neurodegenerative diseases, and metabolic disorders. Unlocking the mechanisms of CLPP activity can provide insights into potential therapeutic targets for diseases associated with mitochondrial dysfunction.
Antibody Name:
Anti-CLPP Antibody
Antibody SKU:
CAB9127
Antibody Size:
20uL, 50uL, 100uL
Application:
WB
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human CLPP (NP_006003.1).
The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.
UniProt Protein Function:
Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
NCBI Summary:
The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
Western blot analysis of extracts of various cell lines, using CLPP antibody (CAB9127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
