The CDK3 Polyclonal Antibody (PAC061514) is a vital tool for researchers studying CDK3, a key protein involved in cell cycle regulation and cell division. This rabbit-derived antibody is highly specific for human samples and has been validated for Western blot applications. By targeting the CDK3 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for research in oncology and cell biology.CDK3 is a critical player in cell cycle progression, influencing key processes such as DNA replication and cell division.
Aberrant expression or dysregulation of CDK3 has been linked to various cancers and other proliferative disorders, making it an attractive target for therapeutic interventions. By understanding the function and regulation of CDK3, researchers can gain valuable insights into the mechanisms of tumorigenesis and potentially identify new treatment strategies for cancer patients.
Antibody Name:
CDK3 Antibody (PACO61514)
Antibody SKU:
PACO61514
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human Cyclin-dependent kinase 3 protein (220-297AA)
IHC image of PACO61514 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.
Synonyms:
Cyclin-dependent kinase 3, Cell division protein kinase 3, CDK3, CDKN3
UniProt Protein Function:
CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.
UniProt Protein Details:
Protein type:Kinase, protein; Cell cycle regulation; Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily
Chromosomal Location of Human Ortholog: 17q25.1
Biological Process: cell proliferation; G0 to G1 transition; G1/S transition of mitotic cell cycle; response to DNA damage stimulus
NCBI Summary:
This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]
