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Canine Stem Cell Factor (SCF) ELISA Kit

SKU:
CNDL00078
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q06220
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
KITLG,MGF,SF,KL1,Kitl,FPH2,Kit-Ligand,Steel Factor,Mast Cell Growth Factor,Steel factor,Familial Progressive Hyperpigmentation 2
Reactivity:
Canine
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Canine Stem Cell Factor (SCF) ELISA Kit

The Canine Stem Cell Factor (SCF) ELISA Kit is specifically designed for the precise measurement of stem cell factor levels in canine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an excellent tool for various research applications.Stem cell factor is a critical factor in canine biology, influencing the growth and differentiation of stem cells.

It plays a crucial role in various biological processes such as hematopoiesis and spermatogenesis, making it a valuable biomarker for studying stem cell function and development in canines. Researchers can utilize this kit to investigate the role of stem cell factor in conditions like cancer, immune disorders, and regenerative medicine, leading to potential therapeutic advancements in veterinary healthcare.

Product Name:Canine Stem Cell Factor (SCF) ELISA Kit
Product Code:CNDL00078
Size:96 Assays
Target:SCF
Synonyms:KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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