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Ca2+-ATPase Activity Assay Kit

SKU:
MAES0213
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue
Sensitivity:
1.18 U/kg wet weight
Range:
1.18-286.43 U/kg
Assay Time:
90 min
$395
Frequently bought together:

Description

Product Name:Ca2+-ATPase Activity Assay Kit
Product Code:MAES0213
Sample Type:Animal Tissue
Assay Time:90 min
Assay Type:Enzyme Activity
Synonyms:Ca2+-ATP
Detection Instrument:Microplate reader (660 nm)
Detection Method:Colorimetric method
Sensitivity:1.18 U/kg wet weight
Detection Range:1.18-286.43 U/kg wet weight
Inter-assay CV:0.08
Intra-assay CV:0.06

ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca2+ -ATPase activity was inhibited, while in the sample system, Ca2+ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca2+ -ATPase during the incubation time. The activity of Ca2+ -ATPase was determined by inorganic phosphorus production.

Storage:2-8°C
Valid Period:12 months
Other Reagents Required:Ultrapure water, Normal saline (0.9% NaCl)
Other Instruments Required:Test tube, Vortex mixer, Incubator, Centrifuge, 100°C water bath, Microplate reader

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