The c-Fos Polyclonal Antibody is a powerful tool for researchers studying the c-Fos protein, a key player in the regulation of gene expression and cell proliferation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the c-Fos protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology, cancer research, and neuroscience.The c-Fos protein is a transcription factor that is rapidly induced in response to various signals, including growth factors, stress, and neuronal activity. Its role in regulating gene expression makes it a crucial target for understanding processes such as cell growth, differentiation, and apoptosis.
With its ability to specifically detect c-Fos, this antibody enables researchers to explore the intricate mechanisms underlying these biological processes and their implications in disease development.By studying the function of the c-Fos protein, researchers can gain valuable insights into the molecular pathways involved in cancer progression, neuronal plasticity, and cellular responses to external stimuli. The c-Fos Polyclonal Antibody is a valuable tool for unlocking the secrets of this essential protein and advancing our understanding of complex biological processes.
Product Name:
c-Fos Polyclonal Antibody
SKU:
CAB21419
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1).
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Purification Method:
Affinity purification
Gene ID:
2353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (CAB21419) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
