The ATP5F1 Polyclonal Antibody (CAB5099) is specifically designed for research involving ATP5F1, a key subunit of mitochondrial ATP synthase involved in energy production in cells. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It specifically binds to the ATP5F1 protein, allowing for precise detection and analysis in various cell types, making it an essential tool for studies in biochemistry and cell biology.ATP5F1, also known as ATP synthase F1 subunit alpha, plays a crucial role in the generation of ATP, the energy currency of the cell. Its involvement in ATP synthesis makes it a vital component of cellular metabolism and energy production processes.
Research into ATP5F1 can provide valuable insights into mitochondrial function, cellular respiration, and energy metabolism, with potential implications for various physiological and pathological conditions.Understanding the function and regulation of ATP5F1 is essential for unraveling the complexities of cellular bioenergetics and mitochondrial physiology. The ATP5F1 Polyclonal Antibody (CAB5099) offers researchers a reliable tool for studying ATP synthase function and exploring its role in cellular processes, making it a valuable asset for investigations in biochemistry, metabolism, and mitochondrial biology.
Product Name:
ATPIF1 Rabbit Polyclonal Antibody
SKU:
CAB5099
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
IP; ATPI; ATPIP; ATPIF1; F1
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
12kDa
Observed MW:
12kDa
Enables several functions, including ATPase binding activity; angiostatin binding activity; and mitochondrial proton-transporting ATP synthase complex binding activity. Involved in several processes, including mitochondrial depolarization; negative regulation of ATPase activity; and regulation of protein targeting to mitochondrion. Located in cell surface and mitochondrion.
Purification Method:
Affinity purification
Gene ID:
93974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and ATPIF1 knockout (KO) 293T(KO) cells, using [KO Validated] ATPIF1 Rabbit pAb (CAB5099) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
