The Vps28 Polyclonal Antibody (CAB19240) is a valuable research tool designed for studying the role of Vps28 in cellular processes, specifically in endosomal sorting and membrane trafficking pathways. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Vps28 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for research in cell biology and molecular biology.Vps28 is a component of the ESCRT-I (endosomal sorting complex required for transport) complex, which plays a crucial role in sorting ubiquitinated membrane proteins for degradation in the lysosome.
Dysregulation of Vps28 and the ESCRT machinery has been implicated in various diseases, including cancer and neurodegenerative disorders. Studying the function of Vps28 using this antibody can provide valuable insights into these disease mechanisms and potentially lead to the development of novel therapeutic strategies targeting the endosomal sorting pathway.
Product Name:
VPS28 Rabbit Monoclonal Antibody
SKU:
CAB19240
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 122-221 of human VPS28 (Q9UK41).
Cell membrane, Late endosome membrane, Peripheral membrane protein.
Calculated MW:
25kDa
Observed MW:
25kDa
This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
51160
Clone Number:
ARC2399
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS28 Rabbit mAb (CAB19240) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
