The Anti-VEGFA Chimeric Recombinant Rabbit Monoclonal Antibody (HDAB0234) offered by Assay Genie is a highly specific and sensitive tool for research involving Vascular Endothelial Growth Factor A (VEGFA). VEGFA is a key regulator of angiogenesis, playing a crucial role in various physiological and pathological processes, including tumor growth and metastasis.This monoclonal antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in applications such as immunohistochemistry and ELISA. By specifically targeting VEGFA, researchers can accurately detect and quantify this important protein in various cell types and tissues, providing valuable insights into angiogenic processes in both normal and disease states.
Studies utilizing the Anti-VEGFA Chimeric Recombinant Rabbit Monoclonal Antibody (HDAB0234) can contribute to a deeper understanding of angiogenesis and aid in the development of novel therapeutic strategies for cancer, cardiovascular diseases, and other conditions where VEGFA plays a critical role. Whether investigating basic mechanisms of angiogenesis or evaluating potential drug targets, this antibody offers reliable and reproducible results for researchers in the fields of oncology, vascular biology, and drug development.
SKU:
HDAB0234
Size:
100 µg
Clonality:
Monoclonal
Clone:
DMC276
Synonyms:
MVCD1, VEGF, VPF
Applications:
Flow Cyt
Recommended Dilution:
Flow Cyt 1:100
Host Species:
Rabbit
Isotype:
Rabbit/Human Fc chimeric IgG1
Reactivity:
Human
Purification Method:
Purified from cell culture supernatant by affinity chromatography
Formulation:
Powder
Buffer:
1XPBS
Storage:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
Usage:
Research use only
Background:
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
