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SARS-CoV-2 NSP10 Rabbit Polyclonal Antibody (CAB20325)

SKU:
CAB20325
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Virus
Host Species:
Rabbit
Isotype:
IgG
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:SARS-CoV-2 NSP10 Rabbit Polyclonal Antibody
SKU:CAB20325
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:SARS-CoV-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of coronavirus NSP10 (YP_009725306.1).
Sequence:AGNA TEVP ANST VLSF CAFA VDAA KAYK DYLA SGGQ PITN CVKM LCTH TGTG QAIT VTPE ANMD QESF GGAS CCLY CRCH IDHP NPKG FCDL KGKY VQIP TTCA NDPV GFTL KNTV CTVC GMWK GYGC SCDQ LREP MLQ
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:2000 - 1:10000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Positive Sample:293T,
Calculated MW:794kDa
Observed MW:20-25kDa/

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Purification Method:Affinity purification
Gene ID:43740578
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of normal 293T cells, 293T transfected with NSP10 Protein and 293T transfected with SOST Protein, using SARS-CoV-2 NSP10 Rabbit pAb (CAB20325) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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