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POLR2C Rabbit Monoclonal Antibody (CAB9744)

SKU:
CAB9744
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
RPB3
RPB31
hRPB33
hsRPB3
Research Area:
Epigenetics and Nuclear Signaling
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:POLR2C Rabbit Monoclonal Antibody
SKU:CAB9744
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPB3/POLR2C (P19387).
Sequence:MPYA NQPT VRIT ELTD ENVK FIIE NTDL AVAN SIRR VFIA EVPI IAID WVQI DANS SVLH DEFI AHRL GLIP LISD DIVD KLQY SRDC TCEE FCPE CSVE
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:RPB3; RPB31; hRPB33; hsRPB3; RPB3/POLR2C
Positive Sample:293T,Mouse kidney,Rat lung,Rat brain,Rat spleen
Conjugate:Unconjugated
Cellular Localization:Cytosol, Nucleoplasm, Nucleus
Calculated MW:31kDa
Observed MW:35kDa

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.

Purification Method:Affinity purification
Gene ID:5432
Clone Number:ARC1729
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using RPB3/POLR2C Rabbit mAb (CAB9744) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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