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PCNT Rabbit Polyclonal Antibody (CAB20161)

SKU:
CAB20161
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
KEN
PCN
MOPD2
PCNT2
PCNTB
PCTN2
SCKL4
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PCNT Rabbit Polyclonal Antibody
SKU:CAB20161
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 95-212 of human PCNT (NP_006022.3).
Sequence:GEKR EDLE QLQQ KQVN DHPP EQCG MFTV SDHP PEQH GMFT VGDH PPEQ RGMF TVSD HPPE QHGM FTVS DHPP EQRG MFTI SDHQ PEQR GMFT VSDH TPEQ RGIF TISD HPAE QRGM FT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4; PCNT
Positive Sample:SH-SY5Y,Rat heart
Conjugate:Unconjugated
Cellular Localization:centriole, centrosome, cytosol
Calculated MW:378kDa
Observed MW:251kDa/378kDa

The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5116
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using PCNT Rabbit pAb (CAB20161) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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