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hnRNP E1/PCBP1 Rabbit Monoclonal Antibody (CAB19276)

SKU:
CAB19276
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
PCBP1
HEL-S-85
HNRPE1
HNRPX
hnRNP-E1
hnRNP-X
poly(rC)-binding protein 1
Research Area:
Epigenetics and Nuclear Signaling
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:hnRNP E1/PCBP1 Rabbit Monoclonal Antibody
SKU:CAB19276
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PCBP1 (Q15365).
Sequence:MDAG VTES GLNV TLTI RLLM HGKE VGSI IGKK GESV KRIR EESG ARIN ISEG NCPE RIIT LTGP TNAI FKAF AMII DKLE EDIN SSMT NSTA ASRP PVTL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; hnRNP E1/PCBP1
Positive Sample:HeLa,HepG2,A-431,K-562,Mouse lung,Mouse brain,Rat pancreas
Conjugate:Unmodified
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:37kDa
Observed MW:40kDa

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Purification Method:Affinity purification
Gene ID:5093
Clone Number:ARC2456
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using hnRNP E1/hnRNP E1/PCBP1 antibody (CAB19276) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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