The EDF1 Polyclonal Antibody (CAB20274) is a valuable tool for researchers studying EDF1, a multifunctional protein involved in various cellular processes. This antibody, generated in rabbits, is highly specific to human samples and has been validated for Western blot applications. It targets the EDF1 protein, allowing for accurate detection and analysis in different cell types.EDF1, also known as endothelial differentiation-related factor 1, is known to play a role in transcriptional regulation, cell proliferation, and differentiation.
Its diverse functions make it a promising target for investigation in the fields of cell biology, developmental biology, and cancer research. Understanding the mechanisms by which EDF1 operates is essential for unraveling its impact on disease pathogenesis and identifying potential therapeutic strategies. Overall, the EDF1 Polyclonal Antibody is a reliable tool for researchers delving into the intricate functions of EDF1 and its potential implications in various biological processes and diseases.
Product Name:
Edf1 Polyclonal Antibody
SKU:
CAB20274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of mouse Edf1 (NP_067494.1).
Predicted to enable TFIID-class transcription factor complex binding activity and transcription coactivator activity. Predicted to be involved in positive regulation of DNA binding activity. Predicted to act upstream of or within cell differentiation. Predicted to be located in cytosol; nucleolus; and nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; heart; liver; and retina. Orthologous to human EDF1 (endothelial differentiation related factor 1).
Purification Method:
Affinity purification
Gene ID:
59022
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Edf1 knockout (KO) NIH/3T3 cells, using [KO Validated] Edf1 Rabbit pAb (CAB20274) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
