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53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00047
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Rat
Detection Method:
Colorimetric
$719
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Description

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53BP1 (Phospho-Ser6)Colorimetric Cell-Based ELISA Kit

The 53BP1 Phospho-Ser6 Colorimetric Cell-Based ELISA Kit is a powerful tool designed for the accurate and sensitive detection of 53BP1 phosphorylated at Ser6 in cell lysates. This kit offers high specificity and sensitivity, enabling researchers to obtain reliable and reproducible results for various research applications.53BP1 is a key protein involved in the DNA damage response pathway, playing a critical role in maintaining genomic stability and DNA repair processes. Phosphorylation of 53BP1 at Ser6 is essential for its function in DNA damage signaling and repair, making it a valuable biomarker for studying cellular responses to genotoxic stress and potential therapeutic interventions.

With its easy-to-use protocol and high-quality components, the 53BP1 Phospho-Ser6 Colorimetric Cell-Based ELISA Kit is a valuable tool for researchers studying DNA damage response pathways, cancer biology, and other related fields. Get accurate and reliable results with this comprehensive kit, advancing your research in understanding the complex mechanisms of DNA damage and repair.

Product Name:53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA
Product Code:CBCAB00047
ELISA Type:Cell-Based
Target:53BP1 (Phospho-Ser6)
Reactivity:Human, Mouse, Rat
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The 53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect 53BP protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated 53BP in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 53BP phosphorylation.

Qualitative determination of 53BP1 (Phospho-Ser6) concentration is achieved by an indirect ELISA format. In essence, 53BP1 (Phospho-Ser6) is captured by 53BP1 (Phospho-Ser6)-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 7158, UniProt ID: Q12888, OMIM: 605230, Unigene: Hs.440968
Gene Symbol:TP53BP1
Sub Type:Phospho
UniProt Protein Function:53BP1: a DNA damage checkpoint protein that interacts with HDAC4 protein to mediate the DNA damage response. Inhibits E2F1-mediated apoptosis in prostate cancer cells. Recruited to double strand breaks via the binding of its Tudor domain to dimethylated H3 K20. Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Binds to the central domain of p53. Interacts with Artemis. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on S139. Interacts with histone H4 that has been dimethylated at K20. Has low affinity for histone H4 containing monomethylated K20. Does not bind histone H4 containing unmethylated or trimethylated K20. Has low affinity for histone H3 that has been dimethylated on K79. Does not bind unmethylated histone H3. Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Two alternatively spliced human isoforms have been observed.
UniProt Protein Details:

Protein type:DNA repair, damage; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function:protein binding; p53 binding; damaged DNA binding; telomeric DNA binding; methylated histone residue binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; double-strand break repair; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination

UniProt Code:Q12888
NCBI GenInfo Identifier:8928568
NCBI Gene ID:7158
NCBI Accession:Q12888.2
UniProt Secondary Accession:Q12888,Q2M1Z7, Q4LE46, Q5FWZ3, Q7Z3U4, F8VY86,
UniProt Related Accession:Q12888
Molecular Weight:1972
NCBI Full Name:Tumor suppressor p53-binding protein 1
NCBI Synonym Full Names:tumor protein p53 binding protein 1
NCBI Official Symbol:TP53BP1  
NCBI Official Synonym Symbols:p202; 53BP1  
NCBI Protein Information:tumor suppressor p53-binding protein 1; p53BP1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
UniProt Protein Name:Tumor suppressor p53-binding protein 1
Protein Family:Tumor suppressor p53-binding protein
UniProt Gene Name:TP53BP1  
UniProt Entry Name:TP53B_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-53BP1 (Phospho-Ser6) Antibody, Anti-53BP1 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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