The ZRANB1 Antibody (PAC062107) is a polyclonal antibody designed for research involving ZRANB1, a protein involved in DNA repair and maintenance. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for studying DNA repair mechanisms. It is validated for use in Western blot applications, enabling detection and analysis of ZRANB1 in various cell types.ZRANB1 plays a critical role in the resolution of DNA replication stress and the maintenance of genomic stability. Mutations in ZRANB1 have been associated with increased susceptibility to cancer, highlighting the importance of understanding its function in DNA repair pathways.
Research on ZRANB1 is essential for developing targeted therapies that exploit DNA repair mechanisms in the treatment of cancer and other genetic disorders.Overall, the ZRANB1 Antibody (PAC062107) is a valuable tool for researchers studying DNA repair processes and their implications in cancer biology, genomics, and personalized medicine. Its high specificity and reactivity with human samples make it an ideal choice for investigating the role of ZRANB1 in maintaining genome stability and preventing the accumulation of mutations that can lead to disease.
Western Blot. Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate. All lanes: ZRANB1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa. Observed band size: 81 kDa.
IHC image of PACO62107 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO62107 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-α signaling.
ZRANB1: Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'- linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling. Belongs to the peptidase C64 family.Protein type: Protease; Ligase; EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 10q26.13Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: protein binding; ubiquitin-specific protease activityBiological Process: cell migration; cytoskeleton organization and biogenesis; positive regulation of Wnt receptor signaling pathway; regulation of cell morphogenesis
