The ZP2 Polyclonal Antibody (CAB10126) is designed for research involving ZP2, a glycoprotein that plays a critical role in the process of sperm-egg recognition and binding during fertilization. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as immunohistochemistry and flow cytometry. It specifically targets the ZP2 protein, enabling precise detection and analysis in reproductive biology research.
ZP2, a member of the zona pellucida protein family, is essential for the formation of the zona pellucida surrounding the oocyte and is involved in sperm binding and fusion. Understanding the function and regulation of ZP2 is crucial for uncovering mechanisms underlying fertilization and infertility. The ZP2 Polyclonal Antibody provides researchers with a valuable tool for studying the biological processes involved in reproductive health and fertility.
Product Name:
ZP2 Rabbit Polyclonal Antibody
SKU:
CAB10126
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 651-745 of human ZP2 (NP_003451.1).
Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space.
Calculated MW:
82kDa
Observed MW:
68kDa
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7783
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ZP2 antibody (CAB10126) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
