The ZMYND8 Polyclonal Antibody is a highly specific research tool designed for the detection of the ZMYND8 protein in samples. ZMYND8 is a key player in epigenetic regulation, functioning as a reader of methylated histones and playing a role in transcriptional regulation.This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blot and immunohistochemistry. Its ability to bind specifically to the ZMYND8 protein allows for accurate detection and analysis in a wide range of cell types, making it an essential tool for researchers studying epigenetics, gene regulation, and cancer biology.
Understanding the function and regulation of the ZMYND8 protein is crucial for unraveling its role in various cellular processes and diseases. With its high specificity and sensitivity, the ZMYND8 Polyclonal Antibody offers a reliable solution for investigating the intricate mechanisms involving ZMYND8 and its potential implications in cancer and other pathological conditions.
Product Name:
ZMYND8 Polyclonal Antibody
SKU:
CAB21848
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RACK7; PRKCBP1; PRO2893; D8
Positive Sample:
HeLa,U-87 MG
Conjugate:
Unconjugated
Cellular Localization:
cytoplasm, Golgi apparatus, nucleoplasm, nucleus
Calculated MW:
132kDa
Observed MW:
Refer to figures
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
23613
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ZMYND8 knockout (KO) HeLa cells, using [KO Validated] ZMYND8 Rabbit pAb (CAB21848) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
