The ZMIZ1 Polyclonal Antibody (PACO59533) is specifically designed for research involving ZMIZ1, a transcriptional coactivator involved in various cellular processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It targets the ZMIZ1 protein, allowing for precise detection and analysis in different cell types, making it ideal for studies in molecular biology and cancer research.ZMIZ1 is known for its role as a transcriptional regulator, playing a key role in gene expression and cellular signaling pathways.
Its involvement in multiple cellular processes makes it a valuable target for research into cancer progression, developmental disorders, and gene regulation mechanisms. Understanding the function of ZMIZ1 is essential for exploring its potential as a therapeutic target in various disease contexts.In conclusion, the ZMIZ1 Polyclonal Antibody (PACO59533) offers researchers a valuable tool for studying the intricate molecular mechanisms involving ZMIZ1, paving the way for advancements in cancer biology and molecular medicine.
IHC image of PACO59533 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO59533 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Acts as transcriptional coactivator. Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. Involved in transcriptional activation of a subset of NOTCH1 target genes including MYC. Involved in thymocyte and T cell development (By similarity).
Synonyms:
Zinc finger MIZ domain-containing protein 1 (PIAS-like protein Zimp10) (Retinoic acid, induced protein 17), ZMIZ1, KIAA1224 RAI17 ZIMP10
UniProt Protein Function:
RAI17: Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressorChromosomal Location of Human Ortholog: 10q22.3Cellular Component: intracellular membrane-bound organelle; nucleoplasm
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the PIAS (protein inhibitor of activated STAT) family of proteins. The encoded protein regulates the activity of various transcription factors, including the androgen receptor, Smad3/4, and p53. The encoded protein may also play a role in sumoylation. A translocation between this locus on chromosome 10 and the protein tyrosine kinase ABL1 locus on chromosome 9 has been associated with acute lymphoblastic leukemia. [provided by RefSeq, Mar 2010]
