The ZFYVE16 Polyclonal Antibody (CAB13154) is a valuable tool for researchers studying the ZFYVE16 protein, which is involved in various cellular processes including endosomal trafficking and autophagy. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.ZFYVE16, also known as Protrudin, is a key player in the regulation of membrane dynamics and vesicle trafficking within the cell. Its role in autophagosome formation and endosomal sorting makes it a crucial target for investigation in the fields of cell biology and neurodegenerative diseases.
By utilizing the ZFYVE16 Polyclonal Antibody, researchers can detect and analyze the expression of the ZFYVE16 protein in different cell types, providing deeper insights into its function and potential implications in disease. This antibody is essential for exploring the molecular mechanisms underlying cellular processes and developing targeted interventions for conditions related to abnormal vesicle transport and autophagy dysfunction.
Product Name:
ZFYVE16 Rabbit Polyclonal Antibody
SKU:
CAB13154
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse ZFYVE16 (NP_775568.1).
Cytoplasm, Early endosome membrane, Peripheral membrane protein.
Calculated MW:
166kDa
Observed MW:
169kDa
Predicted to enable 1-phosphatidylinositol binding activity and phosphatidylinositol-3,4,5-trisphosphate binding activity. Predicted to be involved in endosomal transport and protein targeting to lysosome. Predicted to be located in cytosol and early endosome. Predicted to be active in early endosome membrane. Is expressed in genitourinary system. Orthologous to human ZFYVE16 (zinc finger FYVE-type containing 16).
Purification Method:
Affinity purification
Gene ID:
218441
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using ZFYVE16 Rabbit pAb (CAB13154) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
