The ZFX Polyclonal Antibody (CAB16444) is a valuable tool for researchers studying the ZFX protein, which is an important transcription factor involved in various cellular processes, including cell proliferation and differentiation. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting ZFX in human samples, making it ideal for use in Western blot applications.ZFX has been implicated in numerous cellular functions, including stem cell self-renewal and maintenance of pluripotency. Its dysregulation has been linked to various diseases, particularly in cancer, where it plays a critical role in tumor progression and metastasis.
By targeting ZFX with this antibody, researchers can gain insights into its function and potential as a therapeutic target for cancer treatment.Overall, the ZFX Polyclonal Antibody (CAB16444) is a valuable tool for scientists conducting research in the fields of cancer biology, stem cell research, and developmental biology. Its reliability and specificity make it an essential component for studies aiming to understand the molecular mechanisms underlying cellular processes regulated by ZFX.
Product Name:
ZFX Rabbit Polyclonal Antibody
SKU:
CAB16444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human ZFX (NP_003401.2).
This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
7543
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZFX Rabbit pAb (CAB16444) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
