The ZEB2 Antibody (CAB5705) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene. RRID AB_2766464 Gene ID 9839 Swiss Prot Synonym SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1; ZEB2
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZEB2 Antibody
SKU:
CAB5705
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIP1, SIP-1, ZFHX1B, HSPC082, SMADIP1, ZEB2
Positive Sample:
Mouse liver, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
136kDa
Observed MW:
210kDa
The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene. RRID AB_2766464 Gene ID 9839 Swiss Prot Synonym SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1; ZEB2
Purification Method:
Affinity purification
Gene ID:
9839
RRID:
AB_2766464
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ZEB2 Rabbit pAb (CAB5705) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using ZEB2 Rabbit pAb (CAB5705) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
