The ZBTB33 Polyclonal Antibody (CAB12900) is a valuable tool for researchers studying the ZBTB33 protein, which is known to play a critical role in gene regulation and cellular processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.ZBTB33, also known as Kaiso, is a transcription factor that interacts with specific DNA sequences to regulate gene expression. Its involvement in diverse cellular functions, including cell proliferation, differentiation, and apoptosis, makes it a compelling target for investigations in various fields such as molecular biology, developmental biology, and cancer research.
By specifically binding to the ZBTB33 protein, this polyclonal antibody enables researchers to detect and study its expression and localization in different cell types. Understanding the mechanisms by which ZBTB33 influences gene expression and cellular behavior is essential for unraveling its impact on physiological and pathological processes, paving the way for potential therapeutic interventions targeting ZBTB33-related pathways.
Product Name:
Kaiso/ZBTB33 Rabbit Polyclonal Antibody
SKU:
CAB12900
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso/Kaiso/ZBTB33 (NP_006768.1).
WB,1:1000 - 1:2000 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
ZNF348; ZNF-kaiso; Kaiso/ZBTB33
Positive Sample:
U-87MG,Mouse kidney,Mouse skeletal muscle
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
74kDa
Observed MW:
74-90kDa
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Purification Method:
Affinity purification
Gene ID:
10009
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit pAb (CAB12900) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
