The ZBTB33 Polyclonal Antibody (CAB12856) is a valuable tool for researchers studying ZBTB33, a transcription factor involved in gene regulation and cell differentiation. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the ZBTB33 protein, this antibody allows for the detection and analysis of ZBTB33 expression in various cell types, making it ideal for research in molecular biology and oncology.
ZBTB33, also known as KAISO, is a key player in the regulation of gene expression and cell proliferation. Its role in influencing cellular processes makes it a target of interest in cancer research, as dysregulation of ZBTB33 has been linked to tumorigenesis and metastasis. By studying the function of ZBTB33, researchers can gain insights into its impact on cell growth and survival, potentially leading to the development of targeted therapies for cancer treatment.
Product Name:
Kaiso/ZBTB33 Rabbit Polyclonal Antibody
SKU:
CAB12856
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human Kaiso/Kaiso/ZBTB33 (NP_006768.1).
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Purification Method:
Affinity purification
Gene ID:
10009
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Kaiso/ZBTB33 Rabbit pAb (CAB12856) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
