The ZBP1 Antibody (CAB13899) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for ZBP1 and is validated for use in techniques such as Western blotting. By binding specifically to the ZBP1 protein, this antibody allows for precise detection and analysis of ZBP1 expression in a variety of cell types.ZBP1, also known as DAI, is a cytoplasmic sensor that plays a crucial role in detecting viral nucleic acids and initiating innate immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZBP1 Antibody
SKU:
CAB13899
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAI, DLM1, DLM-1, C20orf183, ZBP1
Positive Sample:
293T transfected with ZBP1, Mouse spleen
Cellular Localization:
Cytoplasm, Cytosol, Nucleus.
Calculated MW:
46kDa
Observed MW:
45kDa/58kDa
This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
81030
RRID
AB_2760753
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using ZBP1 Rabbit pAb (CAB13899) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZBP1 using ZBP1 Rabbit pAb (CAB13899) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
