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YY1 Rabbit Monoclonal Antibody (CAB19569)

SKU:
CAB19569
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:YY1 Rabbit Monoclonal Antibody
SKU:CAB19569
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY1 (P25490).
Sequence:PGNK KWEQ KQVQ IKTL EGEF SVTM WSSD EKKD IDHE TVVE EQII GENS PPDY SEYM TGKK LPPG GIPG IDLS DPKQ LAEF ARMK PRKI KEDD APRT IACP H
Tested Applications:WB IF/ICC IP ChIP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1; YY1
Positive Sample:Mouse liver,Mouse kidney,U-251MG,HeLa,Jurkat,Raji
Conjugate:Unconjugated
Cellular Localization:Nucleus matrix.
Calculated MW:45kDa
Observed MW:60kDa

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Purification Method:Affinity purification
Gene ID:7528
Clone Number:ARC0048
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using YY1 Rabbit mAb (CAB19569) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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