The YWHAE Polyclonal Antibody (CAB1058) is specifically designed for research involving the YWHAE protein, which is part of the 14-3-3 family of proteins known for their role in regulating various cellular processes. The antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.YWHAE is involved in diverse cellular functions, including cell cycle regulation, apoptosis, and signal transduction pathways. It plays a crucial role in mediating protein-protein interactions and is implicated in a wide range of diseases, making it a key target for research in areas such as cancer, neurodegenerative disorders, and metabolic diseases.
By utilizing the YWHAE Polyclonal Antibody, researchers can accurately detect and analyze the YWHAE protein in different cell types, providing valuable insights into its function and potential therapeutic implications. Whether studying cellular signaling pathways or investigating disease mechanisms, this antibody serves as a valuable tool for advancing scientific understanding in various fields of biology and medicine.
Product Name:
14-3-3 epsilon Rabbit Polyclonal Antibody
SKU:
CAB1058
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-163 of human 14-3-3 epsilon (NP_006752.1).
Sequence:
KGGE DKLK MIRE YRQM VETE LKLI CCDI LDVL DKHL IPAA NTGE SKVF YYKM KGDY HRYL AEFA TGND RKEA AENS LVAY KAAS DIAM TEL
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7531
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon antibody (CAB1058) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
