The YWHAB Polyclonal Antibody (CAB1023) is designed for research involving the YWHAB protein, also known as 14-3-3 beta/alpha protein. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the YWHAB protein, this antibody allows for detection and analysis in various cell types, making it an ideal tool for studies in immunology, cell signaling, and cancer research.YWHAB is a multifunctional protein involved in numerous cellular processes, including cell cycle regulation, apoptosis, and signal transduction.
Its role as a scaffolding protein that interacts with a wide range of target proteins makes it a key player in cell signaling networks. Research on YWHAB is important for understanding its impact on cell growth, differentiation, and survival, as well as its potential as a therapeutic target in cancer and other diseases.Overall, the YWHAB Polyclonal Antibody (CAB1023) offers researchers a reliable tool for investigating the functions and interactions of the YWHAB protein in various biological contexts, providing valuable insights into its roles in health and disease.
Product Name:
14-3-3 alpha/beta Rabbit Polyclonal Antibody
SKU:
CAB1023
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-246 of human 14-3-3 alpha/beta (NP_003395.1).
Sequence:
AYQE AFEI SKKE MQPT HPIR LGLA LNFS VFYY EILN SPEK ACSL AKTA FDEA IAEL DTLN EESY KDST LIMQ LLRD NLTL WTSE NQGD EGDA GEGE N
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
7529
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using 14-3-3 alpha/beta Rabbit pAb (CAB1023) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
