The YTHDF1 Polyclonal Antibody (CAB13260) is a valuable tool for researchers studying YTHDF1, a key protein involved in RNA metabolism and gene expression regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting YTHDF1 in human samples, making it suitable for various applications such as Western blot and immunohistochemistry.YTHDF1 is a member of the YTH domain-containing family of proteins, which play important roles in mRNA decay and translation regulation. As a reader protein for N6-methyladenosine (m6A) modifications on RNA molecules, YTHDF1 is involved in various biological processes, including cell differentiation, development, and cancer progression.
Research on YTHDF1 can provide insights into the mechanisms underlying these processes and potentially lead to the development of novel therapeutic strategies.By targeting YTHDF1, researchers can investigate its functions in different cellular contexts and disease states, contributing to a better understanding of RNA metabolism and its implications for human health. The YTHDF1 Polyclonal Antibody (CAB13260) enables precise and reliable detection of YTHDF1 protein levels, facilitating in-depth studies that advance our knowledge of gene expression regulation and its relevance in various biological processes.
Antibody Name:
Anti-YTHDF1 Antibody
Antibody SKU:
CAB13260
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IF
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2).
Application:
WB IF
Recommended Dilution:
WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
HepG2, Jurkat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
YTH domain-containing family protein 1; DACA-1; YTH domain family 1; YTH domain family protein 1; YTH domain family, member 1; dermatomyositis associated with cancer putative autoantigen 1
UniProt Protein Name:
YTH domain-containing family protein 1
UniProt Synonym Protein Names:
Dermatomyositis associated with cancer putative autoantigen 1; DACA-1
Protein Family:
YTH domain-containing family protein
UniProt Gene Name:
YTHDF1Â Â
UniProt Entry Name:
YTHD1_HUMAN
Western blot analysis of extracts of various cell lines, using YTHDF1 antibody (CAB13260) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunofluorescence analysis of L929 cells using YTHDF1 Rabbit pAb (CAB13260) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using YTHDF1 Rabbit pAb (CAB13260) at dilution of 1:100. Blue: DAPI for nuclear staining.
