The YTHDC1 Polyclonal Antibody (CAB7318) is a valuable tool for researchers studying YTHDC1, a key player in RNA metabolism and regulation. This antibody, produced in rabbits, is highly specific and reacts well with human samples, making it ideal for use in Western blot applications. By binding to the YTHDC1 protein, this antibody allows for the detection and analysis of YTHDC1 in various cell types, facilitating research in areas such as RNA processing, gene expression, and epigenetic regulation.YTHDC1 is an RNA-binding protein known for its role in mRNA splicing and processing, as well as its involvement in regulating alternative splicing events. Its functions in post-transcriptional gene regulation make it a crucial player in the control of gene expression and cellular processes.
Research on YTHDC1 is essential for gaining insights into RNA biology, understanding the mechanisms of gene regulation, and exploring the implications of dysregulated RNA metabolism in diseases such as cancer and neurological disorders.With its ability to target and detect YTHDC1, the CAB7318 antibody offers researchers a powerful tool for investigating the functions and mechanisms of this important RNA-binding protein. By elucidating the roles of YTHDC1 in various cellular processes and disease states, scientists can advance our understanding of RNA biology and potentially uncover new therapeutic targets for a range of human disorders.
Product Name:
YTHDC1 Rabbit Polyclonal Antibody
SKU:
CAB7318
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human YTHDC1 (NP_001026902.1).
Sequence:
MAAD SREE KDGE LNVL DDIL TEVP EQDD ELYN PESE QDKN EKKG SKRK SDRM ESTD TKRQ KPSV HSRQ LVSK PL
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:100
Synonyms:
YT521; YT521-B; YTHDC1
Positive Sample:
HeLa,HCT116,293F,RAW264.7,C6,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
85kDa
Observed MW:
110kDa
Enables N6-methyladenosine-containing RNA binding activity. Involved in mRNA export from nucleus; mRNA splice site selection; and regulation of gene expression. Located in nuclear speck and plasma membrane.
Purification Method:
Affinity purification
Gene ID:
91746
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using YTHDC1 antibody (CAB7318) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
