The YAP1 Rabbit Monoclonal Antibody (CAB19134) is a valuable tool for research involving YAP1, a key transcriptional co-activator involved in the Hippo signaling pathway, which plays a crucial role in cell proliferation, organ size control, and tumorigenesis. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.YAP1 is a critical regulator of cellular growth and survival, and dysregulation of its activity has been implicated in the development and progression of various cancers, making it a promising target for cancer research.
By binding specifically to the YAP1 protein, this antibody allows for the detection and analysis of YAP1 expression in different cell types, providing valuable insights into its function and potential therapeutic implications.The significance of YAP1 in cancer biology and its potential as a therapeutic target make this antibody an essential tool for researchers studying cell signaling, oncogenesis, and tumor progression. Understanding the molecular mechanisms involving YAP1 is essential for developing targeted therapies and improving treatment outcomes for cancer patients.
Product Name:
YAP1 Rabbit Monoclonal Antibody
SKU:
CAB19134
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
WB,1:2000 - 1:4000 IHC-P,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
YAP; YKI; COB1; YAP2; YAP-1; YAP65; P1
Positive Sample:
HeLa,Mouse heart,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW:
72kDa
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10413
Clone Number:
ARC53477
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using [KO Validated] YAP1 Rabbit mAb (CAB19134) at1:4000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
