The YAP1 Polyclonal Antibody (CAB11264) is a valuable tool for researchers studying the YAP1 protein, a key regulator of cell growth and apoptosis. This antibody, derived from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the YAP1 protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential tool for studies in cell biology and cancer research.YAP1, also known as Yes-associated protein 1, plays a critical role in controlling cell proliferation and survival by interacting with transcription factors involved in cell growth regulation.
Dysregulation of YAP1 has been implicated in various diseases, including cancer, making it a promising target for therapeutic intervention. Understanding the function of YAP1 is key to developing targeted treatments that can inhibit its activity in diseased cells, highlighting the importance of research using the YAP1 Polyclonal Antibody (CAB11264).
Product Name:
[KO Validated] YAP1 Rabbit Polyclonal Antibody
SKU:
CAB11264
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
YAP; YKI; COB1; YAP2; YAP-1; YAP65; P1
Positive Sample:
A-549,HeLa,BT-474,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
54kDa
Observed MW:
75kDa
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
10413
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] YAP1 Rabbit pAb (CAB11264) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
